Fundamentals of Biology: Lecture Outline - Chapter 6 - Metabolism

Energy:

Potential Energy

Kinetic Energy

Thermal Energy

Chemical Energy

 

- kilocalorie = amount of energy to heat 1000 grams of water from 14.5-15.5 deg. C

 

Cells use energy to -

 

Laws of Thermodynamics

First Law: total amount of energy

 

Second Law of Thermodynamics: spontaneous direction

 

 

Entropy = amount of disorder

 

*Life does not violate this law because the earth is not a "closed system"

Source of energy for life =

One-way flow of energy

 

 

Exergonic "energy out" reactions

 

 

Endergonic "energy in" reactions

 

 

The Role of Adenosine Triphosphate:

- main energy carrier

- "earn" ATP in

- "spend" ATP during

 

ATP/ADP cycle

 

Oxidation/Reduction Reactions:

- electron transfers

Oxidation = removal

Reduction = addition

Metabolic Pathways

 

Chemical equilibrium

- any reversible reaction runs spontaneously toward equilibrium

 

- Note: amount of products and reactants

 

Law of Conservation of Mass

 

Electron Transfer Chains

- enzymes + coenzymes

- one molecule becomes oxidized, the next in line

- electrons enter

 

 

Enzymes:

 

- Speed up

 

- Reused

 

- Act on both the forward + reverse

 

- Very selective

 

 

Mechanisms of Enzyme Action

 

- Help substrates get together

- Orient substrates in correct positions

- Shut out water

- Induce changes

 

"Induced Fit" Model

 

 

Cofactors

- speed up reaction or carry electrons

 

Allosteric Control

- some enzymes have control sites in addition to active sites

 

- Activator - binds to allosteric site

 

- Inhibitor

 

 

Feedback Inhibition

 

- end product inhibits enzyme in pathway

 

Factors Influencing Enzyme Activity

Temperature

pH

 

- pH of stomach = 1-2

 

- pH of small intestine =

 

Beer, Enzymes and your Liver (6.8 Focus on Health)

 

 

Alcoholic hepatitis

Alcoholic cirrhosis

Bioluminescence - a visible sign of metabolism

Enzyme = luciferase

 

 

Clinical application

 

Review - Chapter 6 (p. 112-113)

Review questions 2-11

Self quiz 1-9