Genetics (BIOL-22700)

Laboratory part

Spring 2008

Labs Tu or Wed or Thu  1-3:50 p.m.
CNS 109

instructor for Labs    Tu            W Th Teaching Assistant	Marina Caillaud (mcaillaud@ithaca.edu) Debbie Mahoney (dmahoney@ithaca.edu) Andrew Smith (asmith@ithaca.edu) Lei Mi-Mi (lmi1@ithaca.edu)
Office:	Marina : 165 CNS Debbie Mahoney:  213 CNS Andrew Smith: 155 CNS Lei Mi-Mi: 179 CNS
Office hours:	Caillaud: Tuesday 4-5:30 Mahoney: TBA Smith: TBA

Date (by week)	Topic	Handout/Animations/Instructions
1/22	Lab 1. Mendelian genetics I -Corn Genetics -Exercises in class	Mendel I Lab-KEY
1/29	Lab 2. Mendelian Genetics II -Exercises in class	Mendel 2 Lab-KEY
2/5	Lab 3. Human genetics and Extensions to Mendel -Exercises in class	Extensions lab-KEY
2/12	Lab 4. Linkage and Recombination -Fruit fly genetics -Exercises in class.	Linkage lab-KEY
2/19	Lab 5. Polymorphism in Mitochondrial DNA I -How to master micropipettors -Genomic DNA extraction of cheek cells -PCR to amplify a 440 bp fragment of human mtDNA	Lab 5 handout How to pipet PCR Another PCR Animation
2/26	Lab 6. Polymorphism in Mitochondrial DNA II -Purification of the PCR product -Gel electrophoresis to quantify mtDNA amplified by PCR -Prepare samples for sequencing	Lab 6 handout Gel electrophoresis Sequencing (select Sanger sequencing then Cycle sequencing) Cycle Sequencing
3/4	Lab 7. Polymorphism in Mitochondrial DNA III - Analyses of mtDNA sequences using international databases in the context of human variation and evolution -Start working on your lab report for LAB5-LAB7. This report is due FRIDAY 21ST OF MARCH. If you need help, you can use two resources : the research paper on the genome size in frogs distributed during lab (as an example) and a formal description of how a research paper is organized (CLICK HERE FOR PDF).	Lab 7 handout Guidelines Report The Genography Project The difference between us: Biology and Race
3/11	no lab (spring Break)
3/18	Lab 8. Recombinant DNA Technologies-Characterization of an unknown plasmid vector I -Plasmid DNA extraction	Overview of Labs 8-10 Lab 8 handout
3/25	Lab 9. Recombinant DNA Technologies-Characterization of an unknown plasmid vector II  -Restriction Digest Analysis	Lab 9 handout DNA Restriction
4/1	Lab 10.Recombinant DNA Technologies-Characterization of an unknown plasmid vector III -quiz on all handout for lab 8, 9 and 10 -Transformation in E.coli	Lab 10 handout DNA Transformation Lab 10 Problems KEY
4/8	Lab 11.Recombinant DNA Technologies-Characterization of an unknown plasmid vector IV -Analysis of Transformation Results -Start Report on experiments Lab 8-11	No handout!
4/15	Lab 12. Genetic Engineering -Exercises in class	Lab 12 Problems key
4/22	Lab 13. Exploring Genomes: an introduction to Bioinformatics -NCBI website -Databases for Genomics	Lab 13 handout
4/29	Lab 14.  Student presentations -Poster presentations in 1st floor of CNS -"croissants"  and coffee !!	Instructions for poster presentations

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Page maintained and updated by Marina Caillaud
Last updated April 17, 2008