BIOC-41000 Experimental Biochemistry
Lecture: Wednesday 1-1:50, CNS 302
Discussion: Wednesday 2-4:50, CNS 302
||2nd Block- DIFFERENT
syllabus for this section
|Instructor:||Scott Ulrich, Chemistry Department||Maki Inada, Biology Department|
|Office:||364 CNS||156 CNS
||Thursday noon-1:00, Friday 11-noon|
Reserve in Lab:
Experimental Biochemistry is a new course to expose you to a variety of experimental techniques in biochemistry, team-taught with Professor Maki Inada. In the first section with me, we will cover protein expression, purification and enzymology. Emphasis will be on learning techniques and learning how to design meaningful experiments using those techniques.
Your responsibilities as a student in Experimental Biochemistry:
For each experiment, I’ll hand out materials that describe the goal, describe techniques needed, and how to prepare the reagents required. Due to the small class size, we’ll work collaboratively to plan and execute the experiments, with the assumption that you will become independent over the course of the semester.
Beta-galactosidase- purification and kinetics (4-5 classes)
Purification of Beta-Galactosidase from lacI- E. coli using affinity chromatography,
Assessment of the purity and concentration of enzyme using SDS-PAGE,
Measuring Vmax, Km and kcat of B-Gal using the colorimetric substrate ONPG
Using B-Gal as a reporter gene: bacterial quorum sensing in Agrobacterium tumefaciens
The Abl oncogene and its inhibition by the human cancer drug Gleevec (2-3 classes)
Working out a coupled assay for measuring the catalytic activity of Abl
Measuring the effect of Gleevec on the catalytic rate of Abl – determining the mechanism of inhibition.
Expression and purification of a clinically derived drug-resistant Abl mutant, measurement of the inhibitory effects of Gleevec and a second generation drug, Dasatinib
||100 pts total|
|Formal lab reports
||100 pts total|
|Symposium presentation||25 pts|
Students with disabilities:
In compliance with Section 504 of the Rehabilitation Act of 1973 and the Americans with Disabilities Act, reasonable accommodation will be provided to students with documented disabilities on a case-by-case basis. Students must register with Student Disability Services with the Office of Academic Support Services (607-274-1005, TDD 607-274-7319, firstname.lastname@example.org) and provide appropriate documentation to Ithaca College before any academic adjustment will be provided.
All the work in this class must be your own, unless stated otherwise. For many of the assignments, it is expected that you will discuss ideas with your classmates. Nevertheless, the final work must be your own, and must reflect your own analysis and interpretation.
Confirmed instances of academic misconduct will result in a zero for that assignment/quiz and referral to the school judiciary system. Please refer to the Student Handbook for a detailed description of the policies regarding student academic conduct. If you have a question about what constitutes plagiarism, refer to the following web site: http://www.ithaca.edu/library/htmls/plagiarism.htm
Student input is highly valued and is important to maintain high quality instruction. Course evaluations are mandatory. You may get a ZERO for a homework assignment or an INCOMPLETE IN THE COURSE if not completed by the due date. The evaluation will be submitted to the department Administrative Assistant.